Enhancing Genetic Resistance to DON: Detailed Experiment Protocol (Ito et al., 2012)
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Step-by-Step Genetic Modification Experiment Illustration

Prompt

Generate a detailed visual representation of a scientific process. Illustrate the steps of a genetic modification experiment as follows: 1. Collecting and culturing water samples; 2. Isolation of the ddnA Gene through PCR amplification; 3. Preparation of the cloning vector pKS13S; 4. Insertion of the isolated gene into the vector; 5. Verification of the insertion through restriction enzyme digestion and sequencing; 6. Transformation of the vector into Escherichia coli cells; 7. Transfer of the vector into a binary vector compatible with Agrobacterium tumefaciens; 8. Introduction of the vector into Agrobacterium cells via electroporation; 9. Use of the modified Agrobacterium culture for vacuum infiltration into wheat leaves; 10. Application of vacuum pressure to facilitate DNA penetration into the plant cells; 11. Culturing of the infiltrated tissue and systematic screening to identify successful integration of the ddnA gene. Below this, generate a timetable Gantt chart representing the timeline of the entire experiment.

Created on 2/17/2024 using DALL-E 3 modelReport
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