Optimized Expression of bTGase and 3C Protease in E. Coli

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Constructing pBAD/3C and pBAD/3C/bTGase Expression Plasmids

Prompt

An illustrative image that shows the construction of a plasmid. In the first part, a visual depiction of the pBAD/3C expression plasmid being constructed by cloning the 3C protease gene from B14 rhinovirus into pBAD/HisA, under the control of the araBAD promoter, being introduced into the cloning connector site between Sac I and Eco RI restriction enzymes at 5' and 3' ends respectively. The second part showcases the construction of the pBAD/3C/bTGase expression plasmid. The visual representation shows the T7 promoter and T7 terminator from pET20b (+) plasmid being subcloned into the pBAD/3C plasmid within Pci I and Bsm BI restriction sites at 5' and 3' ends respectively. Finally, the image hints at the gene fragment of bTGase from Bacillus amyloliquefaciens DSM7 and the prodomain of Streptomyces caniferus being cloned into pBAD/3C under the control of the T7 promoter.

Created on 1/21/2024 using DALL·E 3 modelReport

License: Free to use with a backlink to Easy-Peasy.AI

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Constructing pBAD/3C and pBAD/3C/bTGase Expression Plasmids

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