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Liver Tissue Extraction Procedure

Prompt

Create a detailed schematic diagram or flow chart displaying the following procedure: 1. Weighing and cutting 25 grams of liver into small pieces on a petri dish. 2. Washing a small amount of sand thoroughly using soap and water until all soap disappears, followed by rinsing the sand with 5% TCA. 3. Placing the mortar and pestle in an ice bath until cold. 4. Grinding the liver tissue together with the washed sand and 25mL of 10% TCA in a cooled mortar and pestle. 5. Straining the liver tissue after grinding through cheesecloth and collecting the filtrate. 6. Rinsing the mortar and pestle with 5% TCA, pouring the washing through the cheesecloth, collecting the filtrate and discarding the residue. 7. Transferring the filtrate into a 100mL graduated cylinder and recording its volume. 8. Transferring the filtrate into a beaker. 9. Stirring the filtrate, slowly adding 2mL of 95% ethanol per 1mL of the liver tissue extract 2:1. 10. Letting it stand for a few minutes until precipitates flocculates. 11. If no flocculation occurs, adding a pinch of NaCl and placing the container in warm water until a visible precipitate appears. 12. Taking note and recording observations. Preference is for clear labels and steps.

Original Prompt: create a schematic diagram or flow chart for procedure:%0A1.%20Weigh%2025grams%20of%20liver%20and%20cut%20into%20small%20pieces%20on%20a%20petri%20dish.%0A2.%20Wash%20a%20small%20amount%20of%20sand%20thoroughly%20with%20soap%20and%20water.%20Rinse%20with%20water%20repeatedly%20until%20the%20soap%20disappears.%20Then%20rinse%20the%20sand%20with%205%25%20TCA.%0A3.%20Place%20the%20mortar%20and%20pestle%20in%20an%20ice%20bath%20until%20cold.%0A4.%20Grind%20the%20liver%20tissue%20together%20with%20the%20washed%20sand%20and%2025mL%20of%2010%25%20TCA%20at%20the%20same%20time%20in%20a%20cooled%20mortar%20and%20pestle.%0A5.%20Strain%20the%20liver%20tissue%20after%20grinding%20through%20cheesecloth%20and%20collect%20the%20filtrate.%0A6.%20Rinse%20the%20mortar%20and%20pestle%20with%205%25%20TCA%20and%20pour%20the%20washing%20through%20the%20cheesecloth.%20Collect%20the%20filtrate%20and%20discard%20the%20residue.%0A7.%20Transfer%20the%20filtrate%20into%20a%20100mL%20graduated%20cylinder%20and%20then%20record%20its%20volume.%0A8.%20Transfer%20the%20filtrate%20in%20a%20beaker.%0A9.%20Stir%20the%20filtrate,%20slowly%20add%202mL%20of%2095%25%20ethanol%20per%201mL%20of%20the%20tissue%20liver%20extract%202%3A1.%0A10.%20Allow%20to%20stand%20for%20a%20few%20minutes%20until%20precipitates%20flocculates.%0A11.%20If%20no%20flocculation%20occurs,%20add%20a%20pinch%20of%20NaCl%20and%20place%20your%20container%20in%20warm%20water%20until%20a%20visible%20precipitate%20appears.%0A12.%20Note%20and%20record%20observation.

Model: Imagen 4

Created on 2/9/2024 Report

Updated on 8/5/2025

License: Free to use with a backlink to Easy-Peasy.AI

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