Liver Tissue Extraction Procedure
Prompt
Create a detailed schematic diagram or flow chart displaying the following procedure: 1. Weighing and cutting 25 grams of liver into small pieces on a petri dish. 2. Washing a small amount of sand thoroughly using soap and water until all soap disappears, followed by rinsing the sand with 5% TCA. 3. Placing the mortar and pestle in an ice bath until cold. 4. Grinding the liver tissue together with the washed sand and 25mL of 10% TCA in a cooled mortar and pestle. 5. Straining the liver tissue after grinding through cheesecloth and collecting the filtrate. 6. Rinsing the mortar and pestle with 5% TCA, pouring the washing through the cheesecloth, collecting the filtrate and discarding the residue. 7. Transferring the filtrate into a 100mL graduated cylinder and recording its volume. 8. Transferring the filtrate into a beaker. 9. Stirring the filtrate, slowly adding 2mL of 95% ethanol per 1mL of the liver tissue extract 2:1. 10. Letting it stand for a few minutes until precipitates flocculates. 11. If no flocculation occurs, adding a pinch of NaCl and placing the container in warm water until a visible precipitate appears. 12. Taking note and recording observations. Preference is for clear labels and steps.
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